Plasmid
CosmoStrep

Part:BBa_K4590012:Design

Designed by: Beatriz Lauriano de Oliveira   Group: iGEM23_USP-Brazil   (2023-10-02)


Malonate incorporation vector


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2374
    Illegal EcoRI site found at 2824
    Illegal XbaI site found at 2797
    Illegal PstI site found at 2032
    Illegal PstI site found at 2785
    Illegal PstI site found at 7628
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2374
    Illegal EcoRI site found at 2824
    Illegal PstI site found at 2032
    Illegal PstI site found at 2785
    Illegal PstI site found at 7628
    Illegal NotI site found at 1072
    Illegal NotI site found at 1213
    Illegal NotI site found at 9508
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2374
    Illegal EcoRI site found at 2824
    Illegal BglII site found at 2833
    Illegal BglII site found at 5065
    Illegal BglII site found at 5536
    Illegal BglII site found at 6354
    Illegal BamHI site found at 461
    Illegal BamHI site found at 989
    Illegal BamHI site found at 2803
    Illegal XhoI site found at 1592
    Illegal XhoI site found at 2152
    Illegal XhoI site found at 8425
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2374
    Illegal EcoRI site found at 2824
    Illegal XbaI site found at 2797
    Illegal PstI site found at 2032
    Illegal PstI site found at 2785
    Illegal PstI site found at 7628
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2374
    Illegal EcoRI site found at 2824
    Illegal XbaI site found at 2797
    Illegal PstI site found at 2032
    Illegal PstI site found at 2785
    Illegal PstI site found at 7628
    Illegal NgoMIV site found at 52
    Illegal NgoMIV site found at 439
    Illegal NgoMIV site found at 1692
    Illegal NgoMIV site found at 1846
    Illegal NgoMIV site found at 1924
    Illegal NgoMIV site found at 3353
    Illegal NgoMIV site found at 3479
    Illegal AgeI site found at 2137
    Illegal AgeI site found at 4038
    Illegal AgeI site found at 5308
    Illegal AgeI site found at 5648
    Illegal AgeI site found at 7318
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 8848
    Illegal BsaI site found at 9123
    Illegal BsaI site found at 10305
    Illegal SapI site found at 291
    Illegal SapI site found at 525
    Illegal SapI site found at 774
    Illegal SapI site found at 3381
    Illegal SapI.rc site found at 3676


Design Notes

We chose genes either from another species of Streptomyces or from a closely related organism like Rhizobium. We chose a vector that conjugatively adapts well to Streptomyces.


Source

Streptomyces hygroscopicus var. ascomyceticus FK520 biosynthetic gene cluster, partial sequence GenBank: AF235504.1 Rhizobium leguminosarum bv. viciae chromosome complete genome, strain 3841 GenBank: AM236080.1 NCBI:txid173259

References